Leaf proteome analysis of eight Populus xeuramericana genotypes: genetic variation in drought response and in water-use efficiency involves photosynthesis-related proteins.
Identifieur interne : 003574 ( Main/Exploration ); précédent : 003573; suivant : 003575Leaf proteome analysis of eight Populus xeuramericana genotypes: genetic variation in drought response and in water-use efficiency involves photosynthesis-related proteins.
Auteurs : Ludovic Bonhomme [France] ; Romain Monclus ; Delphine Vincent ; Sabine Carpin ; Anne-Marie Lomenech ; Christophe Plomion ; Franck Brignolas ; Domenico MorabitoSource :
- Proteomics [ 1615-9861 ] ; 2009.
Descripteurs français
- KwdFr :
- Analyse de regroupements (MeSH), Caractère quantitatif héréditaire (MeSH), Croisements génétiques (MeSH), Eau (physiologie), Feuilles de plante (métabolisme), Génotype (MeSH), Photosynthèse (génétique), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), Protéines végétales (métabolisme), Protéome (analyse), Sécheresses (MeSH), Variation génétique (MeSH), Électrophorèse bidimensionnelle sur gel (MeSH).
- MESH :
- analyse : Protéome.
- croissance et développement : Populus.
- génétique : Photosynthèse, Populus.
- métabolisme : Feuilles de plante, Populus, Protéines végétales.
- physiologie : Eau.
- Analyse de regroupements, Caractère quantitatif héréditaire, Croisements génétiques, Génotype, Sécheresses, Variation génétique, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- Cluster Analysis (MeSH), Crosses, Genetic (MeSH), Droughts (MeSH), Electrophoresis, Gel, Two-Dimensional (MeSH), Genetic Variation (MeSH), Genotype (MeSH), Photosynthesis (genetics), Plant Leaves (metabolism), Plant Proteins (metabolism), Populus (genetics), Populus (growth & development), Populus (metabolism), Proteome (analysis), Quantitative Trait, Heritable (MeSH), Water (physiology).
- MESH :
- chemical , analysis : Proteome.
- chemical , metabolism : Plant Proteins.
- genetics : Photosynthesis, Populus.
- growth & development : Populus.
- metabolism : Plant Leaves, Populus.
- chemical , physiology : Water.
- Cluster Analysis, Crosses, Genetic, Droughts, Electrophoresis, Gel, Two-Dimensional, Genetic Variation, Genotype, Quantitative Trait, Heritable.
Abstract
Genetic variation of leaf proteome in drought response was investigated among eight Populus xeuramericana genotypes contrasting for their leaf carbon isotope discrimination (Delta), an estimate of intrinsic water-use efficiency. Plants were grown in open field on two similar plots. Drought was induced by an 86-day irrigation cessation on one plot, whereas a second plot remained regularly irrigated. Using 2-DE, 863 reproducible spots were detected; about 60% presented at least one significant effect i.e. treatment, genotype and/or genotype by treatment interaction effect. A significant genotype by treatment interaction was detected for 62 reliably identified proteins among which, about 65% consisted in chloroplast-associated proteins either involved in the Calvin cycle or in the electron-transport chains. The other proteins were involved in oxidative stress, amino acid or protein metabolisms. Correlations between protein abundance and Delta variations were found for 45 reliably identified proteins. The abundance of ribulose-1,5-bisphosphate carboxylase/oxygenase activase isoforms scaled negatively with Delta regardless of the treatment, suggesting that a large intrinsic water-use efficiency could be due to higher abundance of ribulose-1,5-bisphosphate carboxylase/oxygenase activase. Under control condition, abundance of enzymes involved in carbon fixation was also negatively correlated with Delta, whereas abundance of enzymes involved in photorespiration or respiration was positively correlated with Delta.
DOI: 10.1002/pmic.200900047
PubMed: 19722189
Affiliations:
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type="university">Université d'Orléans</orgName></affiliation></author><author><name sortKey="Monclus, Romain" sort="Monclus, Romain" uniqKey="Monclus R" first="Romain" last="Monclus">Romain Monclus</name></author><author><name sortKey="Vincent, Delphine" sort="Vincent, Delphine" uniqKey="Vincent D" first="Delphine" last="Vincent">Delphine Vincent</name></author><author><name sortKey="Carpin, Sabine" sort="Carpin, Sabine" uniqKey="Carpin S" first="Sabine" last="Carpin">Sabine Carpin</name></author><author><name sortKey="Lomenech, Anne Marie" sort="Lomenech, Anne Marie" uniqKey="Lomenech A" first="Anne-Marie" last="Lomenech">Anne-Marie Lomenech</name></author><author><name sortKey="Plomion, Christophe" sort="Plomion, Christophe" uniqKey="Plomion C" first="Christophe" last="Plomion">Christophe Plomion</name></author><author><name sortKey="Brignolas, Franck" sort="Brignolas, Franck" uniqKey="Brignolas F" first="Franck" last="Brignolas">Franck Brignolas</name></author><author><name 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first="Delphine" last="Vincent">Delphine Vincent</name></author><author><name sortKey="Carpin, Sabine" sort="Carpin, Sabine" uniqKey="Carpin S" first="Sabine" last="Carpin">Sabine Carpin</name></author><author><name sortKey="Lomenech, Anne Marie" sort="Lomenech, Anne Marie" uniqKey="Lomenech A" first="Anne-Marie" last="Lomenech">Anne-Marie Lomenech</name></author><author><name sortKey="Plomion, Christophe" sort="Plomion, Christophe" uniqKey="Plomion C" first="Christophe" last="Plomion">Christophe Plomion</name></author><author><name sortKey="Brignolas, Franck" sort="Brignolas, Franck" uniqKey="Brignolas F" first="Franck" last="Brignolas">Franck Brignolas</name></author><author><name sortKey="Morabito, Domenico" sort="Morabito, Domenico" uniqKey="Morabito D" first="Domenico" last="Morabito">Domenico Morabito</name></author></analytic><series><title level="j">Proteomics</title><idno type="eISSN">1615-9861</idno><imprint><date when="2009" 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(MeSH)</term><term>Photosynthèse (génétique)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (métabolisme)</term><term>Protéome (analyse)</term><term>Sécheresses (MeSH)</term><term>Variation génétique (MeSH)</term><term>Électrophorèse bidimensionnelle sur gel (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Proteome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Photosynthesis</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Photosynthèse</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Eau</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Water</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cluster Analysis</term><term>Crosses, Genetic</term><term>Droughts</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Genetic Variation</term><term>Genotype</term><term>Quantitative Trait, Heritable</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de regroupements</term><term>Caractère 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Plants were grown in open field on two similar plots. Drought was induced by an 86-day irrigation cessation on one plot, whereas a second plot remained regularly irrigated. Using 2-DE, 863 reproducible spots were detected; about 60% presented at least one significant effect i.e. treatment, genotype and/or genotype by treatment interaction effect. A significant genotype by treatment interaction was detected for 62 reliably identified proteins among which, about 65% consisted in chloroplast-associated proteins either involved in the Calvin cycle or in the electron-transport chains. The other proteins were involved in oxidative stress, amino acid or protein metabolisms. Correlations between protein abundance and Delta variations were found for 45 reliably identified proteins. The abundance of ribulose-1,5-bisphosphate carboxylase/oxygenase activase isoforms scaled negatively with Delta regardless of the treatment, suggesting that a large intrinsic water-use efficiency could be due to higher abundance of ribulose-1,5-bisphosphate carboxylase/oxygenase activase. Under control condition, abundance of enzymes involved in carbon fixation was also negatively correlated with Delta, whereas abundance of enzymes involved in photorespiration or respiration was positively correlated with Delta.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19722189</PMID><DateCompleted><Year>2009</Year><Month>11</Month><Day>04</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1615-9861</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>17</Issue><PubDate><Year>2009</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Proteomics</Title><ISOAbbreviation>Proteomics</ISOAbbreviation></Journal><ArticleTitle>Leaf proteome analysis of eight Populus xeuramericana genotypes: genetic variation in drought response and in water-use efficiency involves photosynthesis-related proteins.</ArticleTitle><Pagination><MedlinePgn>4121-42</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/pmic.200900047</ELocationID><Abstract><AbstractText>Genetic variation of leaf proteome in drought response was investigated among eight Populus xeuramericana genotypes contrasting for their leaf carbon isotope discrimination (Delta), an estimate of intrinsic water-use efficiency. Plants were grown in open field on two similar plots. Drought was induced by an 86-day irrigation cessation on one plot, whereas a second plot remained regularly irrigated. Using 2-DE, 863 reproducible spots were detected; about 60% presented at least one significant effect i.e. treatment, genotype and/or genotype by treatment interaction effect. A significant genotype by treatment interaction was detected for 62 reliably identified proteins among which, about 65% consisted in chloroplast-associated proteins either involved in the Calvin cycle or in the electron-transport chains. The other proteins were involved in oxidative stress, amino acid or protein metabolisms. Correlations between protein abundance and Delta variations were found for 45 reliably identified proteins. The abundance of ribulose-1,5-bisphosphate carboxylase/oxygenase activase isoforms scaled negatively with Delta regardless of the treatment, suggesting that a large intrinsic water-use efficiency could be due to higher abundance of ribulose-1,5-bisphosphate carboxylase/oxygenase activase. Under control condition, abundance of enzymes involved in carbon fixation was also negatively correlated with Delta, whereas abundance of enzymes involved in photorespiration or respiration was positively correlated with Delta.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bonhomme</LastName><ForeName>Ludovic</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Université d'Orléans, UFR-Faculté des Sciences, Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, Orléans, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Monclus</LastName><ForeName>Romain</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Vincent</LastName><ForeName>Delphine</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Carpin</LastName><ForeName>Sabine</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Lomenech</LastName><ForeName>Anne-Marie</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Plomion</LastName><ForeName>Christophe</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Brignolas</LastName><ForeName>Franck</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Morabito</LastName><ForeName>Domenico</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Proteomics</MedlineTA><NlmUniqueID>101092707</NlmUniqueID><ISSNLinking>1615-9853</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical><Chemical><RegistryNumber>059QF0KO0R</RegistryNumber><NameOfSubstance UI="D014867">Water</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016000" MajorTopicYN="N">Cluster Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003433" MajorTopicYN="Y">Crosses, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055864" MajorTopicYN="Y">Droughts</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="N">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010788" MajorTopicYN="N">Photosynthesis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019655" MajorTopicYN="N">Quantitative Trait, Heritable</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014867" MajorTopicYN="N">Water</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>9</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>9</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>11</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19722189</ArticleId><ArticleId IdType="doi">10.1002/pmic.200900047</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Centre-Val de Loire</li><li>Région Centre</li></region><settlement><li>Orléans</li></settlement><orgName><li>Université d'Orléans</li></orgName></list><tree><noCountry><name sortKey="Brignolas, Franck" sort="Brignolas, Franck" uniqKey="Brignolas F" first="Franck" last="Brignolas">Franck Brignolas</name><name sortKey="Carpin, Sabine" sort="Carpin, Sabine" uniqKey="Carpin S" first="Sabine" last="Carpin">Sabine Carpin</name><name sortKey="Lomenech, Anne Marie" sort="Lomenech, Anne Marie" uniqKey="Lomenech A" first="Anne-Marie" last="Lomenech">Anne-Marie Lomenech</name><name sortKey="Monclus, Romain" sort="Monclus, Romain" uniqKey="Monclus R" first="Romain" last="Monclus">Romain Monclus</name><name sortKey="Morabito, Domenico" sort="Morabito, Domenico" uniqKey="Morabito D" first="Domenico" last="Morabito">Domenico Morabito</name><name sortKey="Plomion, Christophe" sort="Plomion, Christophe" uniqKey="Plomion C" first="Christophe" last="Plomion">Christophe Plomion</name><name sortKey="Vincent, Delphine" sort="Vincent, Delphine" uniqKey="Vincent D" first="Delphine" last="Vincent">Delphine Vincent</name></noCountry><country name="France"><region name="Centre-Val de Loire"><name sortKey="Bonhomme, Ludovic" sort="Bonhomme, Ludovic" uniqKey="Bonhomme L" first="Ludovic" last="Bonhomme">Ludovic Bonhomme</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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